Juventus are aiming for a fourth consecutive Serie A title and third consecutive Europa League triumph after a 2-1 aggregate victory over Udinese at the Stadio Olimpico in Turin.
Juventus have taken a 1-0 lead into the second leg with the hosts leading by just two goals at the break, but the Bianconeri have kept a clean sheet on their last six away matches.
The last time Juventus beat Udinese was in August 2012, when Udinese drew 0-0 at the San Siro.
Juve are aiming to become the first Serie A side to win a game against Udinese in the Champions League group stages.
The last time the two sides met in the competition was at the start of the 2014-15 campaign.
Juveniles goalkeeper David Trezeguet has also played a part in their recent success, keeping a clean record of 16 consecutive clean sheets, while Lazio defender Andrea Ranocchia has netted six goals in eight appearances.
Juvies head coach Roberto Mancini is hoping his side can build on the two-goal first leg win, with the visitors needing to break the deadlock if they are to reach the third round of the competition.
Mancini told reporters: “I think we can achieve this goal with the players who are there and with the intensity we showed tonight.”
It is a fantastic atmosphere and we have a lot of experience in the stadium.
“The first leg was a good start and we will play better.
We have to do better on the counter-attack and the defence.”
If we want to reach third, we have to make it harder for Udinese.
“We need to improve the quality of our players, especially in the second half.”
Injuries are another factor that could affect the game.
Mancinis has named the back line unchanged for the second Leg, but Juventus have not been able to replace Dani Alves, who has been out with a hamstring injury.
Sampdoria, Roma and Juventus all sit in the same group with the latter three facing each other at the end of the tie.